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Размер
- 100 μL
- 200 μL
- 50 μL
Symmetric DiMethyl-Histone H3-R8 Rabbit pAb (A20820)
- Описание
- Характеристики
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Overview
Product name Symmetric DiMethyl-Histone H3-R8 Rabbit pAb Catalog No. A20820 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic symmetric dimethylated peptide around R8 of human histone H3 (NP_003520.1). Sequence QTARK Gene ID Swiss Prot Synonyms H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A Calculated MW 15kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IP ChIP Recommended dilution - WB 1:100 - 1:500
- ChIP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, NIH/3T3, C6, H3 Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R8 antibody (A20820) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Symmetric DiMethyl-Histone H3-R8 antibody (A20820) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic symmetric dimethylated peptide around R8 of human histone H3 (NP_003520.1). Isotype IgG