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Размер
- 100 μL
- 200 μL
- 50 μL
MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)
- Описание
- Характеристики
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Overview
Product name MonoMethyl-Histone H3-K9 Rabbit pAb Catalog No. A2358 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic monomethylated peptide around K9 of human histone H3 (NP_003520.1). Sequence ARKST Gene ID Swiss Prot Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3 Calculated MW 16kDa Observed MW 14kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq DB Recommended dilution - DB 1:500 - 1:2000
- WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- ChIP 1:50 - 1:100
- ChIP-seq 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa, NIH/3T3, C6 Cellular location Chromosome, Nucleus Customer validation WB(HeLa, Mus musculus, Solanum lycopersicum, Musa nana Lour)
IF(Mus musculus)
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K9 antibody (A2358) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (A2358).
Immunohistochemistry analysis of paraffin-embedded human colon using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 antibody (A2358). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-K9 antibody (A2358) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 antibody (A2358) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 antibody (A2358) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K9 antibody (A2358) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K9 antibody (A2358) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic monomethylated peptide around K9 of human histone H3 (NP_003520.1). Isotype IgG