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Размер
- 100 μL
- 200 μL
- 50 μL
MonoMethyl-Histone H4-K20 Rabbit pAb (A2370)
- Описание
- Характеристики
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Overview
Product name MonoMethyl-Histone H4-K20 Rabbit pAb Catalog No. A2370 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.Immunogen information
Immunogen A synthetic monomethylated peptide around K20 of human Histone H4 (NP_003529.1). Sequence HRKVL Gene ID Swiss Prot Synonyms H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A Calculated MW 11kDa Observed MW 11kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC DB Recommended dilution - DB 1:500 - 1:2000
- WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H4 protein Cellular location Chromosome, Nucleus Customer validation WB(Mouse uterus, Mus musculus, Eurosta solidaginis, Homo sapiens)
IF(Mus musculus)
Co-IP(Mus musculus)
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-K20 antibody (A2370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-K20 antibody (A2370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H4-K20 antibody (A2370).
Immunohistochemistry analysis of paraffin-embedded human colon using MonoMethyl-Histone H4-K20 antibody (A2370) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using MonoMethyl-Histone H4-K20 antibody (A2370) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using MonoMethyl-Histone H4-K20 antibody (A2370) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 antibody (A2370). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic monomethylated peptide around K20 of human Histone H4 (NP_003529.1). Isotype IgG