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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
TriMethyl-Histone H3-K27 Rabbit pAb (A2363)
- Описание
- Характеристики
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Overview
Product name TriMethyl-Histone H3-K27 Rabbit pAb Catalog No. A2363 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic trimethylated peptide around K27 of human histone H3 (NP_003520.1). Sequence AARKS Gene ID Swiss Prot Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3 Calculated MW 16kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
- ChIP 1:20 - 1:100
- ChIP-seq 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples MCF7 acid extract, 293T acid extract, C2C12 acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein Cellular location Chromosome, Nucleus Customer validation IF(Human embryonic stem cells, Mouse E16.5 neocortex, Goat sperm, HEK293T, Rice, Homo sapiens, Gallus gallus, Mus musculus, Sus scrofa)
ChIP(A549, Mouse embryonic stem cells, HeLa, Human, Rice, Oryza sativa, Gossypium, Rattus norvegicus, B. napus, castor bean, Mus musculus)
IHC(Mouse lung tumor, Sus scrofa, Mus musculus, Homo sapiens)
WB(Homo sapiens, Rattus norvegicus, Mus musculus, Drosophila, Monascus ruber, Danio rerio , Eurosta solidaginis)
ChIP(Homo sapiens, Gossypium, Mus musculus)
IP(Gossypium, Arabidopsis)
ChIP-qPCR(Mus musculus, Homo sapiens)
ChIP-Seq(Zea mays, Homo sapiens, Oryza sativa L)
IP(Mus musculus)
CHIP(castor bean , Homo sapiens, Mus musculus, Arabidopsis thaliana)
ChiP(Mus musculus)
IF(Homo sapiens, Mus musculus, Danio rerio )
CoIP(N. benthamiana)
IHC(Homo sapiens,Mus musculus)
ChIP(Homo sapiens)
CHIP-seq(Brassica napus)
ChIP-qPCR(Danio rerio )
CHIP-qPCR(Fragaria ananassa)
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (A2363).
Immunohistochemistry analysis of paraffin-embedded human testis using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K27 Rabbit pAb (A2363) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 antibody (A2363) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K27 Rabbit pAb antibody (A2363) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic trimethylated peptide around K27 of human histone H3 (NP_003520.1). Isotype IgG