быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
TriMethyl-Histone H3-K4 Rabbit pAb (A2357)
- Описание
- Характеристики
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Overview
Product name TriMethyl-Histone H3-K4 Rabbit pAb Catalog No. A2357 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic trimethylated peptide around K4 of human histone H3 (NP_003520.1). Sequence RTKQT Gene ID Swiss Prot Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3 Calculated MW 16kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IP ChIP ChIP-seq DB Recommended dilution - DB 1:500 - 1:2000
- WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
- ChIP 1:20 - 1:100
- ChIP-seq 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, NIH/3T3, C6, H3, Arabidopsis thaliana (Before inflorescence) Cellular location Chromosome, Nucleus Customer validation WB(Human cells, HEK293T, Mouse Bone marrow stromal cells, Rattus norvegicus, Mus musculus, Homo sapiens, Oryza sativa, Shrimp, Saccharomyces cerevisiae, Drosophila, Danio rerio , Eurosta solidaginis)
ChIP(Plant samples, Mouse primary calvarial cells, Mus musculus, Oryza sativa, Rattus norvegicus, Arabidopsis thaliana, B. napus)
IF(HEK293T, Mouse E16.5 neocortex, Raw264.7, Homo sapiens, Oryza sativa, Mus musculus, Sus scrofa)
IHC(Rattus norvegicus, Mus musculus, Bombyx mori)
IF(Rattus norvegicus)
WB(Rattus norvegicus)
IP(Homo sapiens, Mus musculus)
ChIP-Seq(Zea mays)
ChIP(Oryza sativa)
ChIP(Homo sapiens)
CHIP(Zea mays, Homo sapiens, Arabidopsis thaliana, Mus musculus)
ChIP-seq(Brassica napus)
CHIP-qPCR(Fragaria ananassa)
Western blot analysis of various lysates, using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of Arabidopsis thaliana (Before inflorescence), using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (A2357) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit pAb antibody (A2357) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic trimethylated peptide around K4 of human histone H3 (NP_003520.1). Isotype IgG