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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
DiMethyl-Histone H3-K36 Rabbit pAb (A2365)
- Описание
- Характеристики
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Overview
Product name DiMethyl-Histone H3-K36 Rabbit pAb Catalog No. A2365 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic dimethylated peptide around K36 of human histone H3 (NP_003520.1). Sequence GVKKP Gene ID Swiss Prot Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3 Calculated MW 16kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
- ChIP 1:20 - 1:100
- ChIP-seq 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa, NIH/3T3, C6, H3 Cellular location Chromosome, Nucleus Customer validation WB(Hippocampus, HeLa, Homo sapiens, Oryza sativa, Penicillium oxalicum)
Western blot analysis of HeLa, using DiMethyl-Histone H3-K36 antibody (A2365) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of various lysates, using DiMethyl-Histone H3-K36 antibody (A2365) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (A2365).
Immunohistochemistry analysis of paraffin-embedded human breast using DiMethyl-Histone H3-K36 antibody (A2365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using DiMethyl-Histone H3-K36 antibody (A2365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using DiMethyl-Histone H3-K36 antibody (A2365) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody (A2365). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody (A2365) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic dimethylated peptide around K36 of human histone H3 (NP_003520.1). Isotype IgG