быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
pan-Symmetric-Di-Methyl Arginine Rabbit pAb (A18261)
- Описание
- Характеристики
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Overview
Product name pan-Symmetric-Di-Methyl Arginine Rabbit pAb Catalog No. A18261 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The methylation of arginine residues in proteins is a post-translational modification that contributes to a wide range of biological processes. Many cytokines involved in T cell development and activation utilize the common cytokine receptor γ-chain (γc) and the kinase JAK3 for signal transduction.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing succinylated K. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 22-70kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver Cellular location Customer validation WB(Mus musculus)
Western blot analysis of extracts of various cell lines, using pan Symmetric-DiMethyl-Arginine antibody (A18261) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using pan Symmetric-DiMethyl-Arginine antibody (A18261) at 1:1000 dilution.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic peptide corresponding to a sequence containing succinylated K. Isotype IgG