быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Pan DiMethyl-lysine Rabbit pAb (A5870)
- Описание
- Характеристики
-
Overview
Product name Pan DiMethyl-lysine Rabbit pAb Catalog No. A5870 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Methylation is a major post-translational modification (PTM) generated by methyltransferase on target proteins, protein methylation plays important regulatory roles in gene expression, protein activity and stability, and signal transduction. Methylation can occur on specific lysine or arginine residues localized within regulatory domains in both histone and nonhistone proteins, thereby allowing distinguished properties of the targeted protein. Lysine can be methylated to different degrees, including mono-, di-, or trimethylation, which reflects its functional diversity and regulatory complexity compared to other PTMs. Lys9 of histone H3 is mono- or di-methylated by G9A/GLP and tri-methylated by SETDB1 to activate transcription. Tumor suppressor p53 is regulated by methylation of at least four sites. p53-mediated transcription is repressed following mono-methylation of p53 at Lys370 by SMYD2; Di-methylation at the same residue further inhibits p53 by preventing association with 53BP1. Concomitant di-methylation at Lys382 inhibits p53 ubiquitination following DNA damage. Di-methylation at Lys373 by G9A/GLP inhibits p53-mediated apoptosis and correlates with tri-methylation of histone H3 Lys9 at the p21 promoter.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing dimethylated K. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 15-60kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:100
- IF/ICC 1:50 - 1:100
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa acid extract, C6 acid extract, H3 protein Cellular location Customer validation WB(Homo sapiens)
Western blot analysis of extracts of various cell lines, using Pan DiMethyl-lysine antibody (A5870) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Dot-blot analysis of all sorts of methylation peptides using Pan DiMethyl-lysine antibody (A5870) at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using Pan DiMethyl-lysine antibody (A5870) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Pan DiMethyl-lysine antibody (A5870) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Pan DiMethyl-lysine antibody (A5870) at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic peptide corresponding to a sequence containing dimethylated K. Isotype IgG