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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
DiMethyl-Histone H3-K14 Rabbit pAb (A5278)
- Описание
- Характеристики
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Overview
Product name DiMethyl-Histone H3-K14 Rabbit pAb Catalog No. A5278 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic dimethylated peptide around K14 of human Histone H3 (NP_003520.1). Sequence GGKAP Gene ID Swiss Prot Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3 Calculated MW 16kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IF/ICC IP ChIP ChIP-seq Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
- ChIP 1:20 - 1:100
- ChIP-seq 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa, NIH/3T3, C6 Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody (A5278) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (A5278) at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic dimethylated peptide around K14 of human Histone H3 (NP_003520.1). Isotype IgG