быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Symmetric DiMethyl-Histone H3-R17 Rabbit pAb (A3152)
- Описание
- Характеристики
-
Overview
Product name Symmetric DiMethyl-Histone H3-R17 Rabbit pAb Catalog No. A3152 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic symmetric dimethylated peptide around R17 of human histone H3 (NP_003520.1). Sequence APRKQ Gene ID Swiss Prot Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J Calculated MW 15kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IF/ICC Recommended dilution - WB 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, NIH/3T3, C6, H3 Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R17 antibody (A3152) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody (A3152).
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 antibody (A3152). Blue: DAPI for nuclear staining.
-
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic symmetric dimethylated peptide around R17 of human histone H3 (NP_003520.1). Isotype IgG