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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Asymmetric DiMethyl-Histone H3-R26 Rabbit pAb (A2375)
- Описание
- Характеристики
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Overview
Product name Asymmetric DiMethyl-Histone H3-R26 Rabbit pAb Catalog No. A2375 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic asymmetric dimethylated peptide around R26 of human histone H3 (NP_003520.1). Sequence AARKS Gene ID Swiss Prot Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J Calculated MW 15kDa Observed MW 14kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, C2C12 Cellular location Chromosome, Nucleus Customer validation WB(Eurosta solidaginis)
Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R26 antibody (A2375) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 150s.
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R26 antibody (A2375). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic asymmetric dimethylated peptide around R26 of human histone H3 (NP_003520.1). Isotype IgG