![[KD Validated] βIII-Tubulin Mouse mAb (A18132)](/upload/resize_cache/iblock/a53/340_340_140cd750bba9870f18aada2478b24840a/xtw2n9abg35yi0mjcnav7svs8tg1bex3.jpg "[KD Validated] βIII-Tubulin Mouse mAb (A18132)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] βIII-Tubulin Mouse mAb (A18132)
- Описание
- Характеристики
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Overview
Product name [KD Validated] βIII-Tubulin Mouse mAb Catalog No. A18132 Host species Mouse Purification method Affinity purification Isotype IgG2b, kappa CloneNo. AMC0115 Background
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human TUBB3 (Q13509). Sequence SDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF Gene ID Swiss Prot Synonyms CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A Calculated MW 50kDa Observed MW 55kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Cytoplasm, cytoskeleton 
Western blot analysis of extracts from wild type(WT) and βIII-Tubulin knockdown (KD) HeLa cells, using βIII-Tubulin antibody (A18132) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of mouse brain cells using [KO Validated] βIII-Tubulin Mouse mAb (A18132) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human TUBB3 (Q13509). Isotype IgG2b, kappa