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Размер
- 100 T
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ABflo® 488 Rabbit anti-Human CD27 mAb (A22063)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human CD27 mAb Catalog No. A22063 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC54542-ABf488 Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-192 of human CD27 (NP_001233.2). Sequence TPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRI Gene ID Swiss Prot Synonyms T14; S152; Tp55; TNFRSF7; S152. LPFS2 Calculated MW 29kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Membrane, Single-pass type I membrane protein 
Flow cytometry:1X10^6 HEL cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).
Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test, right).
Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009，5 μl/Test).
Flow cytometry:1X10^6 Monkey PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009，5 μl/Test).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-192 of human CD27 (NP_001233.2). Isotype IgG