![[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)](/upload/resize_cache/iblock/fe6/340_340_140cd750bba9870f18aada2478b24840a/86ycwd9w41pxl009wmsgxgxs4unpsfgu.jpg "[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706)
- Описание
- Характеристики
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Overview
Product name [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb Catalog No. A22706 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58291 Background
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.Immunogen information
Immunogen Recombinant protein of human Integrin alpha 5 (ITGA5/CD49e). Sequence Email for sequence Gene ID Swiss Prot Synonyms FNRA; CD49e; VLA-5; VLA5A Calculated MW 115kDa Observed MW 150kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, A-549 Cellular location Membrane, Single-pass type I membrane protein 
Western blot analysis of extracts from A-549 cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (A22706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (A22706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human Integrin alpha 5 (ITGA5/CD49e). Isotype IgG