[KD Validated] RAB10 Rabbit mAb (A22746)

быстрая доставка ~4 недели

Размер

100 μL
200 μL
50 μL

Описание
Характеристики

Overview

Product name	[KD Validated] RAB10 Rabbit mAb
Catalog No.	A22746
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55653

Background

RAB10 belongs to the RAS (see HRAS; MIM 190020) superfamily of small GTPases. RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking (Bao et al., 1998 [PubMed 9918381]).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 101-200 of human RAB10 (NP_057215.3).
Sequence	SKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWKSKCC
Gene ID
Swiss Prot
Synonyms	RAB10; ras-related protein Rab-10
Calculated MW	23kDa
Observed MW	23kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IF/ICC
Recommended dilution	WB 1:10000 - 1:70000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, U-87MG, MCF7, C2C12, C6
Cellular location	Cell projection, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lipid-anchor, Recycling endosome membrane, cilium, phagosome membrane, trans-Golgi network membrane

Western blot analysis of various lysates, using RAB10 antibody (A22746) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of extracts from wild type(WT) and RAB10 knockdown (KD) U-87MG(KD) cells, using RAB10 antibody (A22746) at 1:60000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Immunofluorescence analysis of MCF7 using [KD Validated] RAB10 Rabbit mAb (A22746) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using [KD Validated] RAB10 Rabbit mAb (A22746) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Key applications	Western blotting, Immunofluorescence
Host species	Кролик
Antibody type	Primary Antibodies
Reactivity	Человек, Мышь, Крыса
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 101-200 of human RAB10 (NP_057215.3).
Isotype	IgG