быстрая доставка ~4 недели
Размер
- 100 T
- 200 T
- 500 T
ABflo® 488 Rabbit anti-Cat CD27 mAb (A23171)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Cat CD27 mAb Catalog No. A23171 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58370-ABf488 Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.Immunogen information
Immunogen Recombinant protein of cat CD27. Sequence ATLAPQCCPEKHYWAQGQLCCQMCKPGTFLVKDCDRHGEAAQCDPCIPGASFSPDHHIRRHCESCRHCNSGLLIRNCTLTANAECACPKGWQCRDKECTECDPPSNPSLTPRPPQAPGPHPQPTHLPYAKKMPEAGTVRQVQTLADFRWLPAPALSTHGPPQRSLCSTDCIR Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Cat Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location External side of plasma membrane 
Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Cat CD27 mAb(A23171, 5 μl/Test, orange line) or ABflo®488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Cat CD27 mAb(A23171, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Кошка Immunogen Recombinant protein of cat CD27. Isotype IgG