![[KD Validated] EGFR Rabbit PolymAb® (A24388)](/upload/resize_cache/iblock/82b/340_340_140cd750bba9870f18aada2478b24840a/wuh7vtoddkfr1ed34o9976wp35x3rf2r.jpg "[KD Validated] EGFR Rabbit PolymAb® (A24388)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] EGFR Rabbit PolymAb® (A24388)
- Описание
- Характеристики
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Overview
Product name [KD Validated] EGFR Rabbit PolymAb® Catalog No. A24388 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC61438_ARC60682 Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.Immunogen information
Immunogen Recombinant protein of human EGFR. Sequence Email for sequence Gene ID Swiss Prot Synonyms EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA; epidermal growth factor receptor Calculated MW 44kDa/69kDa/77kDa/134kDa Observed MW 145-175kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Cell membrane, Single-pass type I membrane protein 
estern blot analysis of extracts from normal (control) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit PolymAb® (A24388) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Immunofluorescence analysis of A-431 using [KD Validated] EGFR Rabbit PolymAb® (A24388) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human EGFR. Isotype IgG