быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Ubiquityl-Histone H2A-K119 Rabbit pAb (A17952)
- Описание
- Характеристики
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Overview
Product name Ubiquityl-Histone H2A-K119 Rabbit pAb Catalog No. A17952 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.H2A clustered histone (H2AC) consists of several H2A family members, which function as replication-dependent histone proteins.Immunogen information
Immunogen A synthetic ubiquitinated peptide around K119 of human HIST1H2AB (NP_734466.1). Sequence LPKKT Gene ID Swiss Prot Synonyms HIST1H2AB Calculated MW 14kDa Observed MW 23kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples C6 Cellular location extracellular exosome, nucleus 
Western blot analysis of extracts of C6 cells, using Ubiquityl-Histone H2A-K119 Rabbit pAb (A17952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic ubiquitinated peptide around K119 of human HIST1H2AB (NP_734466.1). Isotype IgG