быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
K6-linkage Specific Polyubiquitin Rabbit pAb (A18106)
- Описание
- Характеристики
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Overview
Product name K6-linkage Specific Polyubiquitin Rabbit pAb Catalog No. A18106 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair. it has been reported that modification at Lys-6 inhibits ubiquitin-dependent protein degradation, a failure of the ubiquitin-proteasome system could play a role in initiating the formation of degradation-resistant PHF tangles.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K6 linkage). Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Mouse, Rat Tested applications WB DB Recommended dilution - WB 1:500 - 1:2000
- DB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples RAW264.7, C6 Cellular location 
Western blot analysis of extracts of various cell lines, using K6-linkage Specific Polyubiquitin Rabbit pAb (A18106) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Dot-blot analysis of all sorts of peptides using K6-linkage Specific Polyubiquitin antibody (A18106) at 1:1000 dilution.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K6 linkage). Isotype IgG