быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
pan-Nitrific-Y Rabbit pAb (A20441)
- Описание
- Характеристики
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Overview
Product name pan-Nitrific-Y Rabbit pAb Catalog No. A20441 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing myristylated K. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 10-250kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, C6, Jurkat, NIH/3T3 Cellular location 
Western blot analysis of extracts of various cell lines, using pan-Nitrific-Y antibody (A20441) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of NIH/3T3 cells, using pan-Nitrific-Y antibody (A20441) at 1:1000 dilution.NIH3T3 cells were treated by Perxynitrate（3mM）for 3 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic peptide corresponding to a sequence containing myristylated K. Isotype IgG