быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Pan-Succinyl-K Rabbit pAb (A20503)
- Описание
- Характеристики
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Overview
Product name Pan-Succinyl-K Rabbit pAb Catalog No. A20503 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Succinylation of protein is a kind of post-translational modification of protein mainly occurring on lysine residues. Compared with methylation and acetylation, succinylation of lysine has a greater effect on protein structure and function. Sirt5 is an important de-succinylation modifying enzyme involved in the regulation of a variety of biological processes, such as reactive oxygen species defense, fatty acid metabolism and cell apoptosisImmunogen information
Immunogen A synthetic peptide corresponding to a sequence containing Succinyl-K. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 10-150kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, MCF7, C6, Mouse liver, Mouse heart Cellular location 
Western blot analysis of extracts of various cell lines, using Pan-Succinyl-K antibody (A20503) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence containing Succinyl-K. Isotype IgG