быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
K63-linkage Specific Ubiquitin Rabbit mAb (A2703)
- Описание
- Характеристики
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Overview
Product name K63-linkage Specific Ubiquitin Rabbit mAb Catalog No. A2703 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2652 Background
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A synthesized peptide derived from human K63-linkage Specific Ubiquitin. Sequence Email for sequence Gene ID Swiss Prot Synonyms HEL-S-50 Calculated MW 26kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat Tested applications IHC-P IF/ICC Recommended dilution - IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Immunohistochemistry Immunofluorescence Positive samples Cellular location Cytoplasm, Nucleus 
Immunohistochemistry analysis of paraffin-embedded human esophagus using K63-linkage Specific Ubiquitin Rabbit mAb (A2703) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using K63-linkage Specific Ubiquitin Rabbit mAb (A2703) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using K63-linkage Specific Ubiquitin Rabbit mAb (A2703) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using K63-linkage Specific Ubiquitin Rabbit mAb (A2703) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using K63-linkage Specific Ubiquitin Rabbit mAb (A2703) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using K63-linkage Specific Ubiquitin Rabbit mAb (A2703) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthesized peptide derived from human K63-linkage Specific Ubiquitin. Isotype IgG