быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827)
- Описание
- Характеристики
-
Overview
Product name Hydroxyl-Histone H2A-Y39 Rabbit mAb Catalog No. A4827 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0253 Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.Immunogen information
Immunogen A synthetic hydroxylated peptide around Y39 of human Histone H2A (P04908). Sequence KGNYS Gene ID Swiss Prot Synonyms H2A.1; H2A.2; H2A/a; H2AC4; H2AFA; HIST1H2AE Calculated MW 14kDa Observed MW 17kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, 293T, Mouse thymus, Rat liver Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic hydroxylated peptide around Y39 of human Histone H2A (P04908). Isotype IgG