быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-ACC1-S79 Rabbit pAb (AP0298)
- Описание
- Характеристики
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Overview
Product name Phospho-ACC1-S79 Rabbit pAb Catalog No. AP0298 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around S79 of human ACC1 (NP_942133.1). Sequence SMSGL Gene ID Swiss Prot Synonyms ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha Calculated MW 266kDa Observed MW 280kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-431, RAW264.7 Cellular location Cytoplasm Customer validation WB(Rattus norvegicus, Mus musculus, Sus scrofa, Homo sapiens)
WB IHC(Homo sapiens)
Western blot analysis of A-431, using Phospho-ACC1-S79 Rabbit pAb (AP0298) at 1:700 dilution.A-431 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Western blot analysis of RAW264.7, using Phospho-ACC1-S79 Rabbit pAb (AP0298) at 1:700 dilution.RAW264.7 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S79 of human ACC1 (NP_942133.1). Isotype IgG