быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-AURKA-T288 Rabbit pAb (AP0523)
- Описание
- Характеристики
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Overview
Product name Phospho-AURKA-T288 Rabbit pAb Catalog No. AP0523 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around T288 of human AURKA (NP_003591.2). Sequence RTTLC Gene ID Swiss Prot Synonyms AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47 Calculated MW 46kDa Observed MW 48kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, HT-29 Cellular location Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole Customer validation WB(Homo sapiens)
Western blot analysis of extracts of HeLa and HT-29 cells, using Phospho-AURKA-T288 antibody (AP0523) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours.HeLa cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around T288 of human AURKA (NP_003591.2). Isotype IgG