быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-α-Synuclein-Y136 Rabbit pAb (AP0254)
- Описание
- Характеристики
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Overview
Product name Phospho-α-Synuclein-Y136 Rabbit pAb Catalog No. AP0254 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around Y136 of human alpha-Synuclein (NP_000336.1). Sequence QDYEP Gene ID Swiss Prot Synonyms PD1; NACP; PARK1; PARK4 Calculated MW 14kDa Observed MW 18kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples Mouse brain Cellular location Cell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, synapse 
Western blot analysis of extracts of Mouse brain, using Phospho-α-Synuclein-Y136 antibody (AP0254) at 1:1000 dilution.Mouse brain were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around Y136 of human alpha-Synuclein (NP_000336.1). Isotype IgG