быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
pan Phospho-Serine/Threonine Mouse mAb (AP1067)
- Описание
- Характеристики
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Overview
Product name pan Phospho-Serine/Threonine Mouse mAb Catalog No. AP1067 Host species Mouse Purification method Affinity purification Isotype IgG2b, kappa CloneNo. AMC0265 Background
Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Global alteration of signal transduction by inhibition of serine/threonine dephosphorylation has recently been shown to markedly potentiate cancer cell killing by the DNA-methylating drug, temozolomide.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing phosphorylated Serine/Threonine. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat Cellular location Customer validation WB(Mus musculus)
Western blot analysis of extracts of Jurkat cells, using pan Phospho-Serine/Threonine antibody (AP1067) at 1:500 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic peptide corresponding to a sequence containing phosphorylated Serine/Threonine. Isotype IgG2b, kappa