быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZNF296 Rabbit pAb (A14633)
- Описание
- Характеристики
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Overview
Product name ZNF296 Rabbit pAb Catalog No. A14633 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II and spermatogenesis. Predicted to act upstream of or within negative regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ZNF296 (NP_660331.1). Sequence MSRRKAGSAPRRVEPAPAANPDDEMEMQDLVIELKPEPDAQPQQAPRLGPFSPKEVSSAGRFGGEPHHSPGPMPAGAALLALGPRNPWTLWTPLTPNYPDRQPWTDKHPDLLTCGRCLQTFPLEAITAFMDHKKLGCQLFRGPSRGQGSEREELKALSCLRCGKQFTVAWKLLRHAQWDHGLSIYQTESEAPEAPLLGLA Gene ID Swiss Prot Synonyms ZFP296; ZNF342 Calculated MW 51kDa Observed MW 56kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, Jurkat, 293T, Mouse kidney, Mouse testis Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using ZNF296 antibody (A14633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ZNF296 (NP_660331.1). Isotype IgG