быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
YKT6 Rabbit pAb (A17084)
- Описание
- Характеристики
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Overview
Product name YKT6 Rabbit pAb Catalog No. A17084 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene product is one of the SNARE recognition molecules implicated in vesicular transport between secretory compartments. It is a membrane associated, isoprenylated protein that functions at the endoplasmic reticulum-Golgi transport step. This protein is highly conserved from yeast to human and can functionally complement the loss of the yeast homolog in the yeast secretory pathway.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-198 of human YKT6 (NP_006546.1). Sequence EFSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM Gene ID Swiss Prot Synonyms YKT6 Calculated MW 22kDa Observed MW 22kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat, A-431 Cellular location apical dendrite, basal dendrite, cytoplasm, cytosol, endoplasmic reticulum, endosome, Golgi apparatus, mitochondrion, plasma membrane, SNARE complex 
Western blot analysis of extracts of various cell lines, using YKT6 antibody (A17084) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-198 of human YKT6 (NP_006546.1). Isotype IgG