быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ABCA8A Rabbit pAb (A20496)
- Описание
- Характеристики
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Overview
Product name ABCA8A Rabbit pAb Catalog No. A20496 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to enable ABC-type xenobiotic transporter activity and lipid transporter activity. Predicted to be involved in several processes, including cholesterol efflux; positive regulation of cholesterol efflux; and sphingomyelin biosynthetic process. Predicted to be located in endoplasmic reticulum and plasma membrane. Predicted to be active in intracellular membrane-bounded organelle. Is expressed in telencephalon. Orthologous to human ABCA8 (ATP binding cassette subfamily A member 8).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 690-828 of mouse ABCA8A (NP_694785.3). Sequence LKCAGSSLFLKKKWGIGYHLSLQLSETCVHERITSLVKQHIPDSKLSAESEGKLSYILPLERTNKFPDLYRDLERSPDLGIENYGVSITTLTEVFLKLEGKSSIDQSDIGMTEDVQAGGARSPERFAEVEQLVSLLNGR Gene ID Swiss Prot Synonyms Calculated MW 184kDa Observed MW 180-250kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Mouse heart, Rat lung, Rat liver Cellular location basolateral plasma membrane, endoplasmic reticulum, plasma membrane 
Western blot analysis of extracts of various cell lines, using ABCA8A antibody (A20496) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded mouse lung using ABCA8A Rabbit pAb (A20496) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 690-828 of mouse ABCA8A (NP_694785.3). Isotype IgG