быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZFPM1 Rabbit pAb (A18183)
- Описание
- Характеристики
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Overview
Product name ZFPM1 Rabbit pAb Catalog No. A18183 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and transcription corepressor activity. Involved in platelet formation; regulation of definitive erythrocyte differentiation; and regulation of gene expression. Part of transcription repressor complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ZFPM1 (NP_722520.2). Sequence MSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPEAPSPPSADVNSPPPLPPPTSPGGPKELEGQEPEPRPTEEEPGSPWSGPDELEPVVQDGQRRIRARLSLATGLSWGPFHGSVQTRASSPRQAEPSPALTLLLVDEACWLRTLPQALTEAEANTEIHRKDDALWCRVTKPVPAGGLLSVLLTAEPHSTPGHPVKKEPAEPTCPAPAHDLQLLPQQAGMASI Gene ID Swiss Prot Synonyms FOG; FOG1; ZNF408; ZNF89A; ZC2HC11A Calculated MW 105kDa Observed MW 105kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples K-562, HEL, Mouse spleen Cellular location nucleoplasm, nucleus 
Western blot analysis of various lysates, using ZFPM1 antibody (A18183) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunofluorescence analysis of C6 cells using ZFPM1 Rabbit pAb (A18183) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ZFPM1 (NP_722520.2). Isotype IgG