[One Step] HSP70 Antibody Kit (RK05659)

Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using HSP70/HSPA1 antibody (A0284) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded rat kidney using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of U2OS cells using HSP70/HSPA1 antibody (A0284). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using HSP70/HSPA1 antibody (A0284) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using HSP70/HSPA1 Rabbit pAb (A0284) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.