![[One Step] Flotillin 1 Antibody Kit (RK05647)](/upload/resize_cache/iblock/d04/340_340_140cd750bba9870f18aada2478b24840a/wg4sjh2z35evoz4h2n7sypdk0pbfgpey.jpg "[One Step] Flotillin 1 Antibody Kit (RK05647)")
быстрая доставка ~4 недели
Размер
- 50 μL
[One Step] Flotillin 1 Antibody Kit (RK05647)
- Описание
- Характеристики
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Overview
Product name [One Step] Flotillin 1 Antibody Kit Catalog No. RK05647 Host species Purification method Isotype Background
Immunogen information
Immunogen Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications Recommended dilution Storage buffer Key application Positive samples Cellular location 
Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts from normal (control) and Flotillin 1 knockout (KO) 293T cells, using Flotillin 1 antibody (A6220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various lysates, using Flotillin 1 antibody (A6220) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat heart using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Flotillin 1 Rabbit pAb (A6220) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Flotillin 1 antibody (A6220). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (A6220) at a dilution of 1:1000.
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Antibody type Primary Antibodies