![[One Step] eIF5A Antibody Kit (RK05645)](/upload/resize_cache/iblock/d04/340_340_140cd750bba9870f18aada2478b24840a/wg4sjh2z35evoz4h2n7sypdk0pbfgpey.jpg "[One Step] eIF5A Antibody Kit (RK05645)")
быстрая доставка ~4 недели
Размер
- 50 μL
[One Step] eIF5A Antibody Kit (RK05645)
- Описание
- Характеристики
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Overview
Product name [One Step] eIF5A Antibody Kit Catalog No. RK05645 Host species Purification method Isotype Background
Immunogen information
Immunogen Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications Recommended dilution Storage buffer Key application Positive samples Cellular location 
Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using eIF5A antibody (A2016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded human breast cancer using eIF5A Rabbit pAb (A2016) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using eIF5A antibody (A2016). Blue: DAPI for nuclear staining.
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Antibody type Primary Antibodies