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[One Step] CTNNA1 Antibody Kit (RK05640)

быстрая доставка ~4 недели

Размер

50 μL

Описание
Характеристики

Overview
Product name [One Step] CTNNA1 Antibody Kit
Catalog No. RK05640
Host species
Purification method
Isotype
Background
Immunogen information
Immunogen
Sequence Email for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Applications
Reactivity
Tested applications
Recommended dilution
Storage buffer
Key application
Positive samples
Cellular location
Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using α-Catenin antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts from normal (control) and α-Catenin Rabbit pAb knockout (KO) 293T cells, using α-Catenin Rabbit pAb antibody (A5635) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat kidney using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human vermiform appendix using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using α-Catenin antibody (A5635) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg α-Catenin antibody (A5635). Western blot was performed from the immunoprecipitate using α-Catenin antibody (A5635) at a dilution of 1:500.