- 50 μL
- Описание
- Характеристики
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Overview
Product name [One Step] Cystatin C Antibody Kit Catalog No. RK05639 Host species Purification method Isotype Background
Immunogen information
Immunogen Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications Recommended dilution Storage buffer Key application Positive samples Cellular location 
Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of Hep G2, using Cystatin C Rabbit pAb (A1561) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using Cystatin C antibody (A1561). Blue: DAPI for nuclear staining.
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Antibody type Primary Antibodies







