быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ZNF517 Rabbit pAb (A15979)
- Описание
- Характеристики
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Overview
Product name ZNF517 Rabbit pAb Catalog No. A15979 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 70-180 of human ZNF517 (NP_998770.2). Sequence EEPGALILQVAEQSVAKASLCTDSRMEAGIMESPLQRKLSRQAGLPGTVWGCLPWGHPVGGHPAPPHPHGGPEDGSDKPTHPRAREHSASPRVLQEDLGRPVGSSAPRYRC Gene ID Swiss Prot Synonyms ZNF517 Calculated MW 55kDa Observed MW 56kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples K-562 Cellular location Nucleus 
Western blot analysis of extracts of K-562 cells, using ZNF517 antibody (A15979) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 70-180 of human ZNF517 (NP_998770.2). Isotype IgG