быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ABCA1 Rabbit pAb (A16337)
- Описание
- Характеристики
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Overview
Product name ABCA1 Rabbit pAb Catalog No. A16337 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1170-1350 of human ABCA1 (NP_005493.2). Sequence DHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQ Gene ID Swiss Prot Synonyms TGD; ABC1; CERP; ABC-1; HDLDT1; HPALP1; HDLCQTL13 Calculated MW 254kDa Observed MW 254kDa Applications
Reactivity Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Mouse heart Cellular location Membrane, Multi-pass membrane protein Customer validation WB(Mus musculus)
Western blot analysis of Mouse heart, using ABCA1 Rabbit pAb (A16337) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using ABCA1 Rabbit pAb (A16337) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1170-1350 of human ABCA1 (NP_005493.2). Isotype IgG