быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
γ-Catenin Rabbit pAb (A0963)
- Описание
- Характеристики
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Overview
Product name γ-Catenin Rabbit pAb Catalog No. A0963 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human γ-Catenin (NP_002221.1). Sequence MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQ Gene ID Swiss Prot Synonyms PG; DP3; PDGB; PKGB; CTNNG; DPIII Calculated MW 82kDa Observed MW 82kDa Applications
Reactivity Human, Mouse Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples MCF7, 293T, Mouse lung Cellular location Cell junction, Cytoplasm, Membrane, Peripheral membrane protein, adherens junction, cytoskeleton, desmosome Customer validation WB(human)
Western blot analysis of extracts of various cell lines, using γ-Catenin antibody (A0963) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg γ-Catenin antibody (A0963). Western blot was performed from the immunoprecipitate using γ-Catenin antibody (A0963) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human γ-Catenin (NP_002221.1). Isotype IgG