![[KD Validated] TOLLIP Rabbit pAb (A21551)](/upload/resize_cache/iblock/a94/340_340_140cd750bba9870f18aada2478b24840a/p2q18477704ju3h6idorh882wrbcsb1q.jpg "[KD Validated] TOLLIP Rabbit pAb (A21551)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] TOLLIP Rabbit pAb (A21551)
- Описание
- Характеристики
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Overview
Product name [KD Validated] TOLLIP Rabbit pAb Catalog No. A21551 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2). Sequence MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP Gene ID Swiss Prot Synonyms IL-1RAcPIP Calculated MW 30kDa Observed MW 30kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, SW620, Mouse testis, Mouse brain, Rat testis Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using TOLLIP antibody (A21551) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3S.
Western blot analysis of extracts from wild type (WT) and TOLLIP knockdown (KD) 293T cells, using TOLLIP antibody (A21551) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3S. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2). Isotype IgG