![[KD Validated] PROM1 Rabbit pAb (A21701)](/upload/resize_cache/iblock/7c7/340_340_140cd750bba9870f18aada2478b24840a/ot2613ne23glwawstcn94dqhogf5b1bj.jpg "[KD Validated] PROM1 Rabbit pAb (A21701)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] PROM1 Rabbit pAb (A21701)
- Описание
- Характеристики
-
Overview
Product name [KD Validated] PROM1 Rabbit pAb Catalog No. A21701 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human PROM1 (NP_006008.1). Sequence ANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSY Gene ID Swiss Prot Synonyms RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061 Calculated MW 97kDa Observed MW 120kDa Applications
Reactivity Human, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
- IP 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Immunofluorescence Positive samples HT-29, HCT116 Cellular location Apical cell membrane, Cell projection, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Multi-pass membrane protein, cilium, microvillus membrane, photoreceptor outer segment 
Western blot analysis of extracts of HT-29 cells, using PROM1 antibody (A21701) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts from wild type(WT) and PROM1 knockdown (KD) HCT116 cells, using PROM1 antibody (A21701) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CD133 Rabbit pAb (A21701) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using CD133 Rabbit pAb (A21701) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat lung using CD133 Rabbit pAb (A21701) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using CD133 antibody (A21701) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HT-29 cells using PROM1 antibody (A21701) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 3 μg CD133 antibody (A21701). Western blot was performed from the immunoprecipitate using CD133 antibody (A21701) at a dilution of 1:1000.
-
Key applications Western blotting, Immunohistochemistry, Immunoprecipitation, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human PROM1 (NP_006008.1). Isotype IgG