![[KD Validated] MAD1/MAD1L1 Rabbit pAb (A22000)](/upload/resize_cache/iblock/78f/340_340_140cd750bba9870f18aada2478b24840a/aegixsf8hiz4lth0xlvgoq15lqj7v5vv.jpg "[KD Validated] MAD1/MAD1L1 Rabbit pAb (A22000)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] MAD1/MAD1L1 Rabbit pAb (A22000)
- Описание
- Характеристики
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Overview
Product name [KD Validated] MAD1/MAD1L1 Rabbit pAb Catalog No. A22000 Host species Rabbit Purification method Affinity purification Isotype IgG Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 351-718 of human MAD1/MAD1L1 (NP_003541.2). Sequence KNSAVTSSARGLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA Gene ID Swiss Prot Synonyms MAD1; MVA7; PIG9; TP53I9; TXBP181 Calculated MW 83kDa Observed MW 83kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, HepG2 Cellular location centrosome, cytoplasm, cytosol, mitotic spindle, mitotic spindle assembly checkpoint MAD1-MAD2 complex, nuclear pore nuclear basket, nucleus, spindle, spindle pole 
Western blot analysis of HepG2, using MAD1/MAD1L1 Rabbit pAb antibody (A22000) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts from wild type(WT) and MAD1/MAD1L1 Rabbit pAb knockdown (KD) 293T cells, using MAD1/MAD1L1 Rabbit pAb antibody (A22000) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 351-718 of human MAD1/MAD1L1 (NP_003541.2). Isotype IgG