![[KD Validated] PGC1α Rabbit pAb (A22113)](/upload/resize_cache/iblock/568/340_340_140cd750bba9870f18aada2478b24840a/l5dmw4ck6qh19gcko6sy5xteu2u877nk.jpg "[KD Validated] PGC1α Rabbit pAb (A22113)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] PGC1α Rabbit pAb (A22113)
- Описание
- Характеристики
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Overview
Product name [KD Validated] PGC1α Rabbit pAb Catalog No. A22113 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 600-700 of human PGC1α (NP_037393.1). Sequence CYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVF Gene ID Swiss Prot Synonyms LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha) Calculated MW 91kDa Observed MW 100kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, SH-SY5Y, Mouse skeletal muscle Cellular location cytoplasm, nucleoplasm, nucleus, PML body 
Western blot analysis of various lysates, using PGC1α Rabbit pAb antibody (A22113) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and PGC1α Rabbit pAb knockdown (KD) HeLa cells, using PGC1α Rabbit pAb antibody (A22113) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human colon using [KO Validated] PGC1α Rabbit pAb (A22113) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] PGC1α Rabbit pAb (A22113) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using [KO Validated] PGC1α Rabbit pAb (A22113) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 600-700 of human PGC1α (NP_037393.1). Isotype IgG