![[KD Validated] MIF Rabbit pAb (A22682)](/upload/resize_cache/iblock/ea6/340_340_140cd750bba9870f18aada2478b24840a/6reeok60q0f063y2w6u284655jesr9pd.jpg "[KD Validated] MIF Rabbit pAb (A22682)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] MIF Rabbit pAb (A22682)
- Описание
- Характеристики
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Overview
Product name [KD Validated] MIF Rabbit pAb Catalog No. A22682 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.Immunogen information
Immunogen Recombinant protein of human MIF. Sequence Email for sequence Gene ID Swiss Prot Synonyms GIF; GLIF; MMIF Calculated MW 12kDa Observed MW 12kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T, U-87MG, Mouse brain Cellular location cell surface, cytoplasm, cytosol, extracellular exosome, extracellular region, extracellular space, nucleoplasm, plasma membrane 
Western blot analysis of various lysates, using MIF antibody (A22682) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and MIF knockdown (KD) 293T(KD) cells, using MIF antibody (A22682) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of U-87MG cells using [KD Validated] MIF Rabbit pAb (A22682) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human MIF. Isotype IgG