![[KD Validated] ME1 Rabbit pAb (A23068)](/upload/resize_cache/iblock/8c7/340_340_140cd750bba9870f18aada2478b24840a/5mvifqwmyzny1j50jgywepc6lpwlek4o.jpg "[KD Validated] ME1 Rabbit pAb (A23068)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] ME1 Rabbit pAb (A23068)
- Описание
- Характеристики
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Overview
Product name [KD Validated] ME1 Rabbit pAb Catalog No. A23068 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.Immunogen information
Immunogen Recombinant protein of human ME1. Sequence IQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ Gene ID Swiss Prot Synonyms MES; HUMNDME Calculated MW 64kDa Observed MW 64kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples MCF7 Cellular location Cytoplasm 
Western blot analysis of extracts from wild type(WT) and ME1 knockdown (KD) MCF7(KD) cells, using ME1 antibody (A23068) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human ME1. Isotype IgG