![[KD Validated] HDAC10 Rabbit pAb (A24335)](/upload/resize_cache/iblock/f84/340_340_140cd750bba9870f18aada2478b24840a/7m5sn2sfgmxfyhh7gb840sjv13h6vk11.jpg "[KD Validated] HDAC10 Rabbit pAb (A24335)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] HDAC10 Rabbit pAb (A24335)
- Описание
- Характеристики
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Overview
Product name [KD Validated] HDAC10 Rabbit pAb Catalog No. A24335 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthesized peptide derived from human HDAC10. Sequence DQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA Gene ID Swiss Prot Synonyms HD10 Calculated MW 71kDa Observed MW 71kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples MCF7, 293T(KD) Cellular location Cytoplasm, Nucleus 
Western blot analysis of MCF7, using HDAC10 Rabbit pAb (A24335) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and HDAC10 knockdown (KD) 293T(KD) cells, using HDAC10 Rabbit pAb (A24335) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthesized peptide derived from human HDAC10. Isotype IgG