![[One Step] Hsp90β antibody Kit (RK05737)](/upload/resize_cache/iblock/d04/340_340_140cd750bba9870f18aada2478b24840a/wg4sjh2z35evoz4h2n7sypdk0pbfgpey.jpg "[One Step] Hsp90β antibody Kit (RK05737)")
быстрая доставка ~4 недели
Размер
- 50 μL
[One Step] Hsp90β antibody Kit (RK05737)
- Описание
- Характеристики
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Overview
Product name [One Step] Hsp90β antibody Kit Catalog No. RK05737 Host species Purification method Isotype Background
Immunogen information
Immunogen Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Applications
Reactivity Tested applications Recommended dilution Storage buffer Key application Positive samples Cellular location Customer validation IHC(Homo sapiens)
WB(Homo sapiens)
Western blot analysis of extracts of HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts from normal (control) and Hsp90β knockout (KO) HeLa cells, using Hsp90β antibody (A1087) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Antibody type Primary Antibodies