быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZFYVE1 Rabbit pAb (A7527)
- Описание
- Характеристики
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Overview
Product name ZFYVE1 Rabbit pAb Catalog No. A7527 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate-containing membranes. This protein contains two zinc-binding FYVE domains in tandem and is reported to localize to the Golgi apparatus. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 500-735 of human ZFYVE1 (NP_067083.1). Sequence AKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKH Gene ID Swiss Prot Synonyms SR3; DFCP1; TAFF1; ZNFN2A1; PPP1R172 Calculated MW 87kDa Observed MW 87kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Endoplasmic reticulum, Golgi apparatus, Golgi stack Customer validation WB(Homo sapiens, Mus musculus)
IF(Homo sapiens)
Co-IP(Mus musculus)
IHC(Homo sapiens)
ICC(Homo sapiens)
Western blot analysis of extracts of 293T cells, using ZFYVE1 antibody (A7527) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 500-735 of human ZFYVE1 (NP_067083.1). Isotype IgG