быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
15-PGDH/HPGD Rabbit pAb (A6926)
- Описание
- Характеристики
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Overview
Product name 15-PGDH/HPGD Rabbit pAb Catalog No. A6926 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human 15-PGDH/15-PGDH/HPGD (NP_000851.2). Sequence MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALVDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGILDPPLIANGLITLIEDDALNGAIMKITTSKGIHFQDYDTTPFQAKTQ Gene ID Swiss Prot Synonyms PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1 Calculated MW 29kDa Observed MW 25kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, THP-1(Negative control) Cellular location Cytoplasm Customer validation IHC(Human breast cancer)
WB(Homo sapiens, Mus musculus)
IHC(Homo sapiens)
Western blot analysis of various lysates, using 15-PGDH/HPGD Rabbit pAb (A6926) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human 15-PGDH/15-PGDH/HPGD (NP_000851.2). Isotype IgG