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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
ZFYVE16 Rabbit pAb (A13154)
- Описание
- Характеристики
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Overview
Product name ZFYVE16 Rabbit pAb Catalog No. A13154 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to enable 1-phosphatidylinositol binding activity and phosphatidylinositol-3, 4, 5-trisphosphate binding activity. Predicted to be involved in endosomal transport and protein targeting to lysosome. Predicted to be located in cytosol and early endosome. Predicted to be active in early endosome membrane. Is expressed in genitourinary system. Orthologous to human ZFYVE16 (zinc finger FYVE-type containing 16).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of mouse ZFYVE16 (NP_775568.1). Sequence MDSYFKAAVSGLDKLLDDFEQNPDKQDYLPDAYAFNQCSVSSESASPQLALLSKDQRCISTCASSEACCEDANETFLEGKIHEGLTSRPNEKNVAGLDLLSSVDASTSDEIQPSCMRRCSKPVCDLISDMGNLVHATNSEEDIKQLLPDDPKSSADTLITLDSSSVSEALTVSSVDCGSNAVREEQNNINAGIKNRDISIKELGVKVDMALFDSCKYNRTENLKDKIISNELETVDFDMPSVLMEQSSEMSNTKDNPQYKRLPCELLKDDGCLAEEKVAVAVNNTECLEEGGGSNTIAMP Gene ID Swiss Prot Synonyms mKIAA0305; B130031L15; B130024H06Rik Calculated MW 166kDa Observed MW 169kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:1000 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cytoplasm, Early endosome membrane, Peripheral membrane protein 
Western blot analysis of extracts of HeLa cells, using ZFYVE16 antibody (A13154) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of mouse ZFYVE16 (NP_775568.1). Isotype IgG